Coding
ArgRS

Part:BBa_K2916000:Design

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-07-19)
Revision as of 11:54, 23 July 2019 by Konstantinos (Talk | contribs) (→‎References)


ArgRS protein equipped with a 10x HIS affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 76
    Illegal SapI.rc site found at 1495


Design Notes

The protein was cloned in the pQE30 vector and expressed in the M15 E.coli strain

Source

Escherichia coli gene alaS

References

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568