Regulatory

Part:BBa_K2624000:Experience

Designed by: Yunge Lou, Yali Wu   Group: iGEM18_CPU_CHINA   (2018-08-22)
Revision as of 21:35, 17 October 2018 by JunQing Flag (Talk | contribs) (→‎Applications of BBa_K2624000)


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Applications of BBa_K2624000

To improve the selectivity of our therapy system, we utilize hTERT, the cancer-specific promoter, and HULC, the HCC-specific promoter, to open an AND-gated system to target HCC (Hepatocellular carcinoma). pGL3-Basic vector, a promoterless vector is used for measuring the activity of promoter with a luciferase assay. We determined the activity of the two promoters by it. Experimental results show that the promoter has a good startup efficiency,although the efficiency is lower than CMV promoter.

20181017205901%21T--CPU_CHINA--hp-qidongzi.png

Figure. The activity of the promoters

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