Coding

Part:BBa_K2729008

Designed by: Hajime Fujita   Group: iGEM18_Tokyo_Tech   (2018-09-30)
Revision as of 02:55, 18 October 2018 by Hajime Fujita (Talk | contribs)


ZsYellow-FMDV 2A

Usage and Biology

ZsYellow is a genetic variant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm. FMDV 2A is "self-cleaving" peptides, and upstream coding region can be a single cistron with the assistance.

Characterization

The sequence is optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the Electrophoresis (Marker: lambda HindIII)


As shown in Figure 1, the band comes between 900 bp to 1,000 bp.

Analysis

Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 367
    Illegal NgoMIV site found at 820
    Illegal AgeI site found at 632
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None