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Part:BBa_K2859000:Experience
Designed by: Kyuhee Jo Group: iGEM18_HAFS (2018-10-10)
Revision as of 22:47, 17 October 2018 by Kyuhee0622 (Talk | contribs)
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UNIQ9a1076356c119c79-partinfo-00000000-QINU UNIQ9a1076356c119c79-partinfo-00000001-QINU
We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel.