Part:BBa_K2640001
Recombinant hCG Beta Subunit Beta 3 Loop
The BBa_K2640001 part is the coding sequence of Recombinant HCG Beta subunit Beta 3 loop epitope. The β3-loop of hCGβ66–80 ,also known as hCG Beta 3 Loop is an epitope present on the beta subunit of the hormone hCG. Human Chorionic Gonadotropin (hCG) is the hormone made by chorionic cells in the fetal part of the placenta where it stimulates the ovaries to synthesize important steroids for the maintenance of pregnancy. It is a member of the beta chain glycoprotein hormone family. The glycoproteins are heterodimers consisting of an alpha subunit and beta subunit. The beta subunit determines bio-specificity. There are numerous epitopes present on the beta subunit of hCG which are detected by hCG antibodies including those present in pregnancy test strips. According to the Journal of Biology Chemistry, “...The performance of a single site assay depends on the identity and nature of the chosen epitope, and it is crucial that it is present, intact and correctly folded in every hCG variant encountered. It is against this background that the β3-loop of hCGβ66–80 has been identified as a potentially ideal epitope…” (Gregor et al, 2011).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Optimized primers for hCG Beta 3 Loop
Name: Beta 3 Loop with biobrick prefix
Sequence: AAGGAGAATTCGCGGCCGCTTCTAGTCCATTCGTTTACCCGGATG
Annealing Temperature: 56℃
Name: Beta 3 Loop Biobrick Suffix
Sequence: AAGGCTGCAGCGGCCGCTACTAGTACACGACTGGGTTTACTCCG
Annealing Temperature: 64℃
Optimized primer for hCG Beta 3 Loop
Name:Beta 3 Loop primer for pGEX F
Sequence: ttcgcccaatttagaattcgAGTCCATTCGTTTACCCGGA v
Annealing Temperature: 68℃
Name: Beta 3 Loop primer for GEX R
Sequence: AGCTCGACGTACGGTCGCGGCCGCACACGACTGGGTTTACTCCGCG
Annealing Temperature: 79℃
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