Translational_Unit

Part:BBa_K914009

Designed by: Jean Cury   Group: iGEM12_Paris_Bettencourt   (2012-09-21)
Revision as of 02:24, 18 October 2018 by Benastles (Talk | contribs)


P1003* Ser133->Amber Codon

The single mutation avoid the expression of the P1003 gene, that prevent the expression of a kanamycin resistance. This mutation is rescue with the tRNA amber suppressor supD (BBa_K914000 - https://parts.igem.org/Part:BBa_K914000), or any genotype relative to sup44 . The idea here is to prevent the kanamycin gene resistance in case horizontal gene transfer. Actually it would be better with another mutation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/semantic_containment
//cds/selectionmarker/antibioticresistance
Parameters
None