Part:BBa_K2876003
Lambda CL-antiAFB1-ScFv2 fusion
A fusion of a Lambda CL repressor and an aflatoxin sensing single chain antibody variable region (antiAFB1-ScFv2). Used in conjunction with BBa_K2876005 (https://parts.igem.org/Part:BBa_K2876005) and BBa_K2876000 (https://parts.igem.org/Part:BBa_K2876000) to create for an E. coli two hybrid system capable of detecting aflatoxin.
For our small molecule test case we chose to detect aflatoxin, a small molecule produced by Aspergillus fungi species that infects many agricultural crops, including cannabis.2,3 Aflatoxin also causes a number of health problems in people when inhaled or ingested by immunocompromised patients.7 We fused two single chain antibodies targeting aflatoxin (Cite BIOBRICK parts) to the lambda repressor and alpha subunit of RNA-polymerase III (Cite BIOBRICK parts) described in Dove, Joung, and Hochschild in order to create two fusion proteins capable of activating transcription in the presence of aflatoxin (Figure 4 small molecule). For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP. See more information about our reporter below.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 715
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1249
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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