Coding

Part:BBa_K2876015

Designed by: Eleanor Glockner   Group: iGEM18_Stanford   (2018-10-17)
Revision as of 02:35, 18 October 2018 by Ijustice (Talk | contribs)


anti-IL1B scFv1

This sequence produces a single-chain antibody that targets the immunoprotein interleukin B1 (BBa_K2876014) (https://parts.igem.org/Part:BBa_K2876014). We fused tthis antibody to the alpha subunit of RNA-polymerase III (BBa_K2876002) (https://parts.igem.org/Part:BBa_K2876002) described in Dove, Joung, and Hochschild in order to a fusion protein capable of activating transcription in the presence of IL1B and another antibody fusion protein. For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP (BBa_K2876000) (https://parts.igem.org/Part:BBa_K2876000). Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 255
    Illegal PstI site found at 288
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 255
    Illegal PstI site found at 288
    Illegal NotI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 756
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 255
    Illegal PstI site found at 288
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 255
    Illegal PstI site found at 288
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/immune_regulation/antibodies
Parameters
None