Coding

Part:BBa_K2876003

Designed by: Isaac Justice   Group: iGEM18_Stanford   (2018-06-22)
Revision as of 07:02, 17 October 2018 by Ijustice (Talk | contribs)


Lambda CL-antiAFB1-ScFv2 fusion

A fusion of a Lambda CL repressor and an aflatoxin sensing single chain antibody variable region (antiAFB1-ScFv2). Used in conjunction with BBa_K2876005 (https://parts.igem.org/Part:BBa_K2876005) and BBa_K2876000 (https://parts.igem.org/Part:BBa_K2876000) to create for an E. coli two hybrid system capable of detecting aflatoxin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 715
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1249
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None