Device

Part:BBa_K2761009

Designed by: Carlos Javier Cordero Oropeza   Group: iGEM18_Tec-Monterrey   (2018-10-10)
Revision as of 20:35, 10 October 2018 by Registry (Talk | contribs)

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Cas1-Cas2 with Flag and Ha tags

Cassette of expression of Cas1 and Cas2 proteins regulated by a T7 promoter induced by IPTG. Contains tags to extract and purified CAS proteins. Cas1 contains a Flag tag and Cas2 contains a HA tag. Each protein contains its own RBS and the construct contains only one T7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 192
    Illegal XhoI site found at 985
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 913
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None