Part:BBa_K775004:Experience
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Applications of BBa_K775004
Improvement of BBa_K775004
iGEM18_NEFU_China creat a new parts，which is based on BBa_K775004. It's BBa_K2542014,and it is more suitable for somewhere which needs low expression in yeast.It's allows a type yeast to sense the alpha factor secreted by alpha-type yeast and express green fluorescent protein. We modified the part BBa_K775004 of the previous team to make the expression efficiency weaker，and make it more appropriate for suicide gene（[http://2018.igem.org/Team:NEFU_China/Suicide Information Destroyed ]）.
User Reviews
UNIQ9f0c45016d7daf60-partinfo-00000000-QINU UNIQ9f0c45016d7daf60-partinfo-00000001-QINU
In the project of 2018, Tsinghua-A team, the part BBa_K775004, the pheromone responsive element pfus1, together with a EGFP reporter gene, was inserted to the plasmid pYES2 which has its original GAL1 promoter removed through HindIII digestion, yielding plasmid pYES2-pfus1-EGFP. pYES2-pfus1-EGFP was transformed into a-typed yeast strain CEN.PK2-1C.
After the addition of α factor, compared with the non-induction group, EGFP intensity inside the yeast got stronger from the 1h group to 5h group, these yeast cells also experience chemotropic growth. 6h after the addition, it seems EGFP protein was released into the medium,as showed in the following figure.See http://2018.igem.org/Team:Tsinghua-A for detailed description.
