Composite

Part:BBa_K2804005

Designed by: Byron Francisco Mosquera   Group: iGEM18_Ecuador   (2018-10-05)
Revision as of 01:12, 18 October 2018 by Pancho Mosquera (Talk | contribs)


CBD cipA fused to sfGFP under the control of LacI promoter

The superfolder green fluorescent protein (sfGFP) is fused to the C-terminus of the cellulose binding domain (CBD) of the scaffolding protein cipA of Clostridium thermocellum. 

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<a name="SF">CBD cipA-sfGFP</a>

<img src="T--Ecuador--CBD-sfGFP.png" width="268" height="261"/>

Figure 2. Tridimensional structure of CBD cipA-sfGFP obtained from the server I-Tasser.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
    Illegal AgeI site found at 1493
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None