Regulatory

Part:BBa_K2560304

Designed by: Benjamin Daniel   Group: iGEM18_Marburg   (2018-10-03)
Revision as of 23:37, 17 October 2018 by Henselt (Talk | contribs)


3-HP Sensor. This part is a PhytoBrick variant


This part contains the promoter as well as the coding sequence for the activator HdpR in the 5´region of the part. It also contains the promotor PHdpR which is regulated by HdpR. This promoter is oriented in reverse to the HdpR codon, facing out of the 3`end of the part. When HdpR binds 3-Hydroxypropionate it changes its conformation, enabling it to bind to PHdpR and activate transcription of the gene controlled by it.


Usage and Biology

  • Inducer: 3-Hydroxypropionate
  • 3-Hydroxypropionate is harmless. For induction, a concentration between 1 and 20 mM can be used.
  • The system stems from the S1 safety strain of P. putida KT2440.
  • We designed this biobrick in order to get a system with a usable readout for the intracellular 3-Hydroxypropionate concentration.
  • Can with be combined with a several reporter genes. We had the best results using the LUX operon.


Figure 1: HIER TEXT
WEITERER TEXT

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1108
    Illegal NotI site found at 279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 263
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/activator
Parameters
None