Composite
Part:BBa_K2680060
Designed by: Stephanie Do, Xiangyi Fang, Ethan Jones, Jessica Laury, Adam Oliver, Lillian Parr, Chengwu Shen, Tinh Son, Julia Urban, Yashna Verma, Hanmi Zou Group: iGEM18_William_and_Mary (2018-10-06)
39C Temperature-Sensitive (ptlpAr) mf-Lon
39C Temperature-Sensitive (ptlpAr) mf-lon. Components: J23107 TPL + ptlpAr BCD8 mf-lon thrL
Mechanics
In this circuit, a temperature-sensitive protein, TLP is expressed constitutively by J23107, a medium-weak constitutive promoter. When the temperature is increased to 39C, TLP looses its ability to repress a downstream pTLPAr promoter. Therefore, temperatures above 39C allow for the expression of mf-Lon.
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Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 51
Illegal NheI site found at 74
Illegal NotI site found at 613 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 898
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1201
Illegal AgeI site found at 3305
Illegal AgeI site found at 3389
Illegal AgeI site found at 3595
Illegal AgeI site found at 3620 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |