Coding
Part:BBa_K2765021:Design
Designed by: Jun Li Group: iGEM18_BIT-China (2018-09-15)
To determine whether overexpression of yno1 can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned yno1 is driven by GAL1 promoter, and thus the target gene is induced by galactose and repressed by glucose.
Obtained gene yno1 though clone from yeast genome.