Coding

Part:BBa_K2657003

Designed by: Kim Ly   Group: iGEM18_Austin_UTexas   (2018-10-09)
Revision as of 22:58, 17 October 2018 by MelodyK (Talk | contribs)


Phytobrick of RCP

In the improved BBa_E1010, specific overhangs for MoClo functional elements were added to the flanks of the part to make it into a PhytoBrick. Additionally, BsaI restriction sites were added so that the improved part could be inserted into the Universal Acceptor.

Figure 2 White circled indicates colonies of expected phenotype.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 572
    Illegal AgeI site found at 684
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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