Composite

Part:BBa_K2533039

Designed by: Ziyi Li   Group: iGEM18_HUST-China   (2018-09-09)
Revision as of 17:03, 17 October 2018 by Zyz2018hust (Talk | contribs)


RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh

It encodes glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b.

Usage and biology

gapA could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate, mdh could transform malate into pyruvate, pflB helps to transform pyruvate into Acetyl-CoA and fdh helps to transform formate into CO2. With the overexpression of gapA-mdh, Shewanella could produce NADH more efficiently, which brings more electricity to be produced.

Characterization

This is the whole circuit for NADH production part.

Figure1:The whole circuit


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Categories
Parameters
chassisShewanella oneidensis
functionproduce glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b.