RNA

Part:BBa_K2725016

Designed by: Eva Steele   Group: iGEM18_Edinburgh_UG   (2018-10-11)
Revision as of 22:10, 17 October 2018 by Evasteele (Talk | contribs)


J23108 - supD - B1006

Serine amber suppressor tRNA gene (supD) downstream of J23108 Anderson promoter. When co-transformed, this part allows the expression of a protein coding gene with up to 5 serine codons substituted for amber stop codons (TAG) - such as BBa_K2725012 - P1003 Kanamycin resistance gene with 5 amber substitutions.

T--Edinburgh_UG--400.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 77
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 80


[edit]
Categories
Parameters
None