Coding

Part:BBa_K2665012

Designed by: You Tong   Group: iGEM18_Kyoto   (2018-10-08)
Revision as of 02:31, 9 October 2018 by TongYou (Talk | contribs) (→‎Characterization)


TDH3-ZrGPD1-6xHis-CYC

ZrGPD1 is the gene that codes a glycerol-3-phosphate dehydrogenase which controls glycerol synthesis in Zygosaccharomyces rouxii. It increases glycerol synthesis which work as compatible solute. So, it may raise osmotolerance in yeast.

Usage and Biology

This part sequence can be used directly because it contains promoter and terminator, but promoter is TDH3 promoter so it can't express in E. coli. If need to use the protein in E. coli, please note that you have to change promoter and terminator. ZrGPD1 is the gene that codes a glycerol-3-phosphate dehydrogenase which controls glycerol synthesis.


Characterization

We used this part in order to enhance the solt tolerance of yeast. We assessed the tolerance in our method(please refer to [http://http://2018.igem.org/Team:Kyoto/Protocol our Wiki's Special Protocol] ).

Result is showed below.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1923
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1454
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1129
    Illegal BsaI site found at 1889
    Illegal BsaI.rc site found at 1271

Reference

[1] Hou,Lihua Wang,Meng Wang,Cong Wang,Chunling Wang,Haiyong (2013) Analysis of salt-tolerance genes in zygosaccharomyces rouxii, Applied Biochemistry and Biotechnoloogy 1417-1425



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