Coding
P1003 5*

Part:BBa_K2725012

Designed by: Eva Steele   Group: iGEM18_Edinburgh_UG   (2018-10-11)
Revision as of 18:50, 11 October 2018 by Registry (Talk | contribs)

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P1003 Cassette with 5 amber stop codons

A P1003 kanamycin resistance cassette with 5 serine -> amber stop codon (TAG) substitutions: S36Amb, S133Amb, S154Amb, S200Amb, S240Amb. This gives kanamycin resistance when co-transformed with BBa_K2725016. This part demonstrates functionality of the semantic containment system for preventing horizontal gene transfer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None