Coding

Part:BBa_K2665011:Experience

Designed by: Masahiro Nanbu   Group: iGEM18_Kyoto   (2018-09-25)
Revision as of 22:03, 17 October 2018 by Nanbu (Talk | contribs)


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Please enter how you used this part and how it worked out.

--Kyoto--mangrin spot.png
T--Kyoto--cont.png

Pictures shown above is colonies of S. cerevisiae ΔENA1- strain on SD midium containing 400mM NaCl. In this spot assay, part BBa_K2665011 cloned to a yeast low-copy vector was used. This result shows that mangrin contributes to salt tolerance of yeasts.


T--Kyoto--K con.jpeg
T--Kyoto--Na con.jpeg
The graph shown above is K+ or Na+ concentration in cells of S.cerevisiae ΔENA1ΔNHA1 strain. Each gene in the graph was cloned to yeast vectors, which were introduced to yeasts. The word “high” means high copy plasmid and the word “low” means low copy plasmids. These transformed yeasts are cultured in SD containing 400mM NaCl. Detailed data are on our wiki.[http://2018.igem.org/Team:Kyoto Kyoto2018] These results show that mangrin also contributes to accumulation of K+ and Na+ in a yeast cell. In addition, mangrin on the high copy plasmid inhibit growth of yeasts too extensively to form colonies.


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