Part:BBa_K2553009

pLuxlacO_superfolderGFP

It is used as the QS signal receptor/reporter module with yemGFP under the control of the synthetic lux promoter PluxlacO to evaluate the synthetic lux system. It can not be found in nature.

It can be used to detect the availability of upstream quorum sensing system. When activated by QS autoinducers, a green fluorescence can be detected as the transcription of GFP is initiated. Using the QS system to control transcription can improve both the efficiency and stability of the target protein's transcription. The autoinducing mechanism saves the effort of using artificial inducers such as IPTG, and reduced steps also mean fewer things can go wrong. Also, this system is self-regulating, which means that the colony will automatically adjust to ensure stable production without the need for interference from researchers.

For those aiming to work with QS system, this construction offers an easy way to evaluate the performance of the system.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 529
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]