Composite
Part:BBa_K2550001:Design
Designed by: Yan Zhang, Janet Standeven, Abby Bell, Megan Hong Group: iGEM18_Lambert_GA (2018-10-08)
T7 promoter with a trigger sequence to match the toehold sequence of part BBa_K2550000
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 104
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
The RNA sequence was obtained from the Georgia Institue of Technology that was number 2 of the 144 generation orthogonal toehold switch collection from the Collins Paper titled, Toehold Switches: De-Novo-Designed Regulators of Gene Expression.
Design Notes
This is meant to be used with toehold part. Needs to be used in BL21 cells that have a T7 polymerase.
Source
Alexander A. Green, Pamela A. Silver, James J. Collins, Peng Yin, Toehold Switches: De-Novo-Designed Regulators of Gene Expression, Cell, Volume 159, Issue 4, 2014, Pages 925-939, ISSN 0092-8674, https://doi.org/10.1016/j.cell.2014.10.002