Tag

Part:BBa_K2719003:Experience

Designed by: Karla Soto Blas   Group: iGEM18_TecCEM   (2018-08-08)
Revision as of 16:44, 17 October 2018 by JRUEDA (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2719003

This part was cloned on pSB1C3 by restriction with EcoRI and PstI and transformed into E.coli DH5a, obtaining white colonies (see figure 1).

T--TecCEM--OPGSTColonies.png

Figure 1. Colonies transformed with optimized GST.

Latter it has extracted and run on an agarose gel electrophoresis (see figure 2).

Finally it was treated with NotI and run on an agarose gel electrophoresis to prove the presence of insert (see figure 3).

User Reviews

UNIQbbc8d187a5dec9b3-partinfo-00000000-QINU UNIQbbc8d187a5dec9b3-partinfo-00000001-QINU