Other

Part:BBa_K2560001:Design

Designed by: Daniel Stukenberg   Group: iGEM18_Marburg   (2018-08-13)
Revision as of 12:24, 13 August 2018 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Phytobrick Entry Vector with RFP dropout


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 21
    Illegal NheI site found at 44
    Illegal SpeI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 51
    Illegal AgeI site found at 630
    Illegal AgeI site found at 742
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 927


Design Notes

More information to come


Source

The Phytobrick Acceptor Vector BBa_P10500 was used as a Template. LacZ was replaced by BBa_E1010 (RFP CDS)using Gibson Assembly

References