Part:BBa_K2888002
fused lysozyme gene
Our composite part within prefix and suffix consists of the most commonly used promoter, RBS, 6x histag and terminator, as well as our functional coding sequence --- cyanophage lysozyme gene. This composite part is assembled through Gibson Assembly; then, it is fused with linearized vector, pSB1C3 backbone, via infusion. This composite part consist of The composite part is utilized to demonstrate the pure effects of lysozyme in our function test--- cyanobacterial lysis reaction. Mainly applied in function test, this composite part without 6x histag was fused with pSB1C3 backbone and then was successfully expressed in E.coli. The lysis reaction was then conducted in the solution with both cultivated cyanobacteria and expressed proteins. In this way, we could examine and quantify the lysis results, confirming the efficacy of cyanophage lysozyme.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 27
Illegal NheI site found at 50 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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