Coding

Part:BBa_K2719004:Experience

Designed by: Karla Soto Blas   Group: iGEM18_TecCEM   (2018-08-08)
Revision as of 20:15, 17 October 2018 by JRUEDA (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2719004

This part was optimized Escherichia coli BL21 (DE3) so it could be transformed into it and also cloned in pSB1C3. For proving it presence it was necessary to make two agarose gels, one for the entire plasmid (figure 2) and the other one for the restriction part in order to observe the insert. (Figure 3)

T--TecCEM--TCSD5Colonies.png

Figure 1. Colonies transformed with Tenascin Fibronectin domains.

T--TecCEM--GelK2719004.png

Figure 2. 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 1)Extraction Tenascin 5 Fibronectin domains, 2)Extraction Tenascin 5 Fibronectin domains duplicate.

User Reviews

UNIQce87afe9efa27c01-partinfo-00000000-QINU UNIQce87afe9efa27c01-partinfo-00000001-QINU