Coding

Part:BBa_K2685006:Design

Designed by: LIN, WENXIN   Group: iGEM18_TUST_China   (2018-10-06)
Revision as of 19:35, 17 October 2018 by YuwenZhang (Talk | contribs)


TetO+TetA

This parts can provide tetracycline tolerance to our constructive detecting chasis which including tetA and tetO genes.

TetA encodes the tetracycline efflux pump protein, which selectively transports tetracycline from thecytoplasm to the periplasm in

exchange for protons, thus limiting tetracycline's access to ribosomes within cells.They are proton power dependent, single polypeptide, drug-specific efflux pump, and it belong to the main promoting factor superfamily.

We also design some single sites mutation comparing with the original sequence that we have got from the biobrick bank.

<img src="T--TUST_China--Parts_document_0.png"/>

Design Notes

Detecting tetracycline and pump out the tetracycline.It is the most important parts in the detection chasis.

Source

TetA is PCR from the biobrick which included in the kit plate.

References