Composite
Part:BBa_K2550001:Design
Designed by: Yan Zhang, Janet Standeven, Abby Bell, Megan Hong Group: iGEM18_Lambert_GA (2018-10-08)
T7 promoter with a trigger sequence to match the toehold sequence of part BBa_K2550000
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 104
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is meant to be used with toehold part. Needs to be used in BL21 cells that have a T7 polymerase.
Source
Collins Paper