Composite

Part:BBa_K2571005:Design

Designed by: Tugba İnanc, Ceyhun Kayihan   Group: iGEM18_METU_HS_Ankara   (2018-07-03)
Revision as of 19:14, 17 October 2018 by Aku2001 (Talk | contribs) (→‎Design Notes)


Bifunctional gamma-glutamate-cysteine ligase/Glutathione synthetase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2069


Design Notes

Design Notes of GSH (BBa_K2571005)

Our circuit consists of prefix, a strong promoter (J23100), RBS (B0034), GSH as protein coding region, double terminator (B0015) and suffix. This part enables our E. coli KO11 strain to overexpress oxidised Glutathione to reduce oxidative stress, increasing its lifespan. (Lu, 2013) Our construct is inserted into pSB1C3 and delivered to the Registry.


Circuit design of BBa_K2571005. Our construct includes a strong promoter,RBS, GSH and double terminator.


In order to make our gene compatible with RFC 10, 25 and 1000, we reconstructed the nucleotides to get rid of the restriction sites while protecting the amino acid sequence. We looked through the codon bias property of E.coli and made the nucleotide changes accordingly.

Source

streptococcus thermophilus

References