Plasmid_Backbone

Part:BBa_K2847002:Design

Designed by: Slavil Peykov   Group: iGEM18_Bulgaria   (2018-10-09)
Revision as of 10:12, 9 October 2018 by Registry (Talk | contribs)

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pSC101 ori + RepA (thermosensitive mutant) + AmpR gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1163
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1163
    Illegal NotI site found at 1482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1163
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1163
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2128


Design Notes

PCR amplification with primers: F-CAGGTTTGTGCCAATACCAG and R-CAGGTGGCACTTTTCGGG


Source

Plasmid pE-FLP (Addgene Plasmid #45978)

References