Composite

Part:BBa_K2557016

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)
Revision as of 15:54, 9 October 2018 by Registry (Talk | contribs)

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TetO-miniCMV promoter-TP901

The TetO sequence, SV40 NLS, flag tag, miniCMV promoter, and TP901 recombinase are ligated in sequence to participate in intracellular signal processing. According to the feedback of the digital model, the different strength promoters upstream of the recombinase and the recombination efficiency of the recombinase will affect the stability of the system. Therefore, in order to optimize our system, we used three promoters and three recombinases, hoping to find the optimal solution.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 391
    Illegal PstI site found at 964
    Illegal PstI site found at 1675
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 964
    Illegal PstI site found at 1675
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 986
    Illegal BamHI site found at 325
    Illegal BamHI site found at 1930
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 391
    Illegal PstI site found at 964
    Illegal PstI site found at 1675
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 391
    Illegal PstI site found at 964
    Illegal PstI site found at 1675
    Illegal NgoMIV site found at 430
    Illegal NgoMIV site found at 574
    Illegal AgeI site found at 331
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1183


[edit]
Categories
//chassis/eukaryote
Parameters
None