Composite
Part:BBa_K2557010
Designed by: Ge JT Group: iGEM18_NAU-CHINA (2018-10-09)
TetO-miniCMV promoter-Bxb1
The TetO sequence, SV40 NLS, flag tag, miniCMV promoter, Bxb1 recombinase are sequentially linked and involved in intracellular signal processing. According to the feedback of the digital model, the different strength promoters upstream of the recombinase and the recombination efficiency of the recombinase will affect the stability of the system. Therefore, in order to optimize our system, we used three promoters and three recombinases, hoping to find the optimal solution.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1099
Illegal BamHI site found at 325
Illegal BamHI site found at 540
Illegal BamHI site found at 856
Illegal BamHI site found at 1564
Illegal BamHI site found at 1855 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 841
Illegal AgeI site found at 331 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 561
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Categories
Parameters
//chassis/eukaryote
None |