![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2557017
Designed by: Ge JT Group: iGEM18_NAU-CHINA (2018-10-09)
TetO-EF1α promoter-TP901
The TetO sequence, SV40 NLS, flag tag, EF1α promoter, and Tp901 recombinase are ligated in sequence, and participate in intracellular signal processing. According to the feedback of the digital model, the different strength promoters upstream of the recombinase and the recombination efficiency of the recombinase will affect the stability of the system. Therefore, in order to optimize our system, we used three promoters and three recombinases, hoping to find the optimal solution.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 565
Illegal PstI site found at 1138
Illegal PstI site found at 1849 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1138
Illegal PstI site found at 1849 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1160
Illegal BamHI site found at 499
Illegal BamHI site found at 2104 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 565
Illegal PstI site found at 1138
Illegal PstI site found at 1849 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 565
Illegal PstI site found at 1138
Illegal PstI site found at 1849
Illegal NgoMIV site found at 604
Illegal NgoMIV site found at 748
Illegal AgeI site found at 505 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1357
[edit]
Categories
Parameters
//chassis/eukaryote
None |