Composite

Part:BBa_K2888010

Designed by: SBS_SH_112144   Group: iGEM18_SBS_SH_112144   (2018-10-07)
Revision as of 05:24, 7 October 2018 by Registry (Talk | contribs)

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fused mlrA gene with histag

This mlrA gene is a part of the microcystin degradation system discovered in Sphingopyxis sp. C-1. In our experiment, we intend to deal with Microcystin-LR, the most common type of microcystin toxin produced in cyanobacteria. The mlrA gene is responsible for hydrolytic cleavage and linearization of cyclic structure of MCLR. We purposely add 6x histag into our sequence in order to improve the efficacy and efficiency of expression and purification; it will also strengthen effects of binding to Ni-NTA column, making it possible to immobilize proteins and lyse MCLR repeatedly in our prototype device.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 770
    Illegal AgeI site found at 305
  • 1000
    COMPATIBLE WITH RFC[1000]


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