Intermediate

Part:BBa_K2771020

Designed by: Felipe Xavier Buson   Group: iGEM18_USP-Brazil   (2018-10-06)
Revision as of 04:06, 7 October 2018 by Registry (Talk | contribs)

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Ratiometric promoter characterization reporter

Combination of EYFP and ECFP to be used in ratiometric characterization of promoters. Both the genes will be exposed to the same external influences (eg. plasmid copy number, culture medium, metabolic burden) and the constitutive expression of ECFP serves as a control for normalization of the EYFP expression, giving the ratiometric output EYFP/ECFP, enhancing the possibility of characterization of intrinsic promoter properties.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None