Part:BBa_K2627000
Advanced CcaS with PAS domain knocked out
CcaS is a protein in CcaS-CcaR two-component system, which is sensitive to green light and red light. CcaS is located on the cell membrane and when it senses the existence of green light or red light, it will experience autophosphorylation and thus phosphorylate the downstream CcaR, and CcaR will bind to specific promoter to activate gene transcription. Typically, gene will be activated under green light and repressed under red light, but this advanced CcaS with PAS domain knocked out has reverse effect, gene will be repressed under green light and activated under red light. In our project, this advanced two-component system is used as a switch to sense different light and turn on the gene to produce PHB.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 606
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1397
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Results
Figure 1: Characterization of part BBa_K2627000 and BBa_K592001. Part BBa_K2627000 shows a different effect that the index under red light is higher than the index under green light. The Fluorescence intensity is about 1207.4 under green light while it is about 3146.3 under red light. For this reason, we can treat the index under red light as expression index and treat the index under green light as leak index.
//chassis/prokaryote/ecoli
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