Composite
NG10

Part:BBa_K2623032

Designed by: Niangui Cai   Group: iGEM18_XMU-China   (2018-10-07)
Revision as of 05:11, 7 October 2018 by Registry (Talk | contribs)

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A composite part linked by BBa_K2623024, BBa_K2623026 and BBa_K2623027

Under the induction of IPTG and Arabinose, this part can code SpyTag-OmpA, SpyCatcher-L7Ae and siRNA-C/Dbox to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatcher and the linkage between L7Ae and C/Dbox.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1965
    Illegal NheI site found at 1988
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3974
    Illegal BamHI site found at 3850
    Illegal BamHI site found at 4004
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
    Illegal AgeI site found at 3685
    Illegal AgeI site found at 4010
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3667


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Categories
Parameters
None