Composite

Part:BBa_K2622033

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)
Revision as of 14:16, 17 October 2018 by GAMBIT (Talk | contribs)


His-igA

His-ScFv-antiVGY has a 6xHis-tag attached to ScFv-antiVGY's (BBa_K2622004) N-terminus. This part could be used to purify ScFv-antiVGY prior to erythrocyte membrane lysis assay or to perform ELISA with ScFv-antiVGY.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 198
    Illegal XbaI site found at 39
    Illegal PstI site found at 181
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 198
    Illegal NheI site found at 108
    Illegal PstI site found at 181
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 198
    Illegal BglII site found at 177
    Illegal BamHI site found at 164
    Illegal XhoI site found at 173
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 198
    Illegal XbaI site found at 39
    Illegal PstI site found at 181
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 198
    Illegal XbaI site found at 39
    Illegal PstI site found at 181
    Illegal NgoMIV site found at 1041
    Illegal AgeI site found at 816
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 20


[edit]
Categories
Parameters
None