Part:BBa_K2533046

RBS-mleS

transport lactate

Usage and biology

lldP refers to L-lactate permease, helping the lactate transported out of the cell. With this gene, Rhodopseudomonas palustris could transport lactate out of the cell more efficiently, which brings Shewanella more carbon sources.

Characterization

This is one section for lactate utilization part.

Figure1:RBS-lldP

DNA Gel Electrophoretic

To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.

Figure2:Verification of successful transformation of pSB1C3-RBS-lldP

Our target genes are 1713bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.

Electrogenesis

By detecting the production of lactate after expressing protein, we might find out whether lldP could effectively help Rhodopseudomonas palustris to transport lactate out of cell.

Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieve the goal to help strains produce lactate.

It could be demonstrated that targeted genes could be expressed in the engineered cells. More lactate has been transported out of the cell by engineered bacteria, which helps to provide more carbon sources for Shewanella.