Part:BBa_K2533031

RBS-lldEFG

It encodes L-lactate dehydrogenase.

Usage and biology

lldEFG refers to three genes: lldE, lldF and lldG. They could encode a L-lactate dehydrogenase complex which could catalyze L-lactate’s transformation into pyruvate.

Characterization

This is one section for lactate utilization part.

Figure1:RBS-lldEFG

DNA Gel Electrophoretic

To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.

Figure2:Verification of successful transformation of pYYDT-lldEFG

Our target genes are 3037bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.

Electrogenesis

By comparing the ability of producing electricity, we might find out whether lldEFG could effectively help Shewanella to produce more electricity.

Figure4:The comparison of electricity production between Shewanella contained pYYDT and pYYDT-lldEFG.

It could be demonstrated that targeted genes could be expressed in the engineered cells. More lactate has been utilized by engineered bacteria, which helps to produce more electricty.