Composite

Part:BBa_K2533002:Design

Designed by: Ziyang Xiao   Group: iGEM18_HUST-China   (2018-09-08)
Revision as of 14:27, 17 October 2018 by Xzy1012 (Talk | contribs) (→‎Design Notes)


RBS-ldhDnARSdR-RBS-lldP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 780
    Illegal BglII site found at 1325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1461
    Illegal AgeI site found at 1924
    Illegal AgeI site found at 2362
    Illegal AgeI site found at 2521
    Illegal AgeI site found at 2533
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 764
    Illegal BsaI.rc site found at 1035


Design Notes

ldhDnARSdR is ldhD (lactate dehydrogenase gene) with D176A/I177R/F178S/N180R. LldP ​​protein gene is used to transport the lactate out of the cell. We successfully construct genes ldhDnARSdR-lldp using molecular biology experiment methods.

Source

From Lactobacillus bulgaricus.

References