Coding

Part:BBa_K2558003

Designed by: Tianze Huang   Group: iGEM18_Tsinghua   (2018-10-02)
Revision as of 13:00, 2 October 2018 by Registry (Talk | contribs)

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dCas9

Cas9 is the main protein of the CRISPR/Cas system II of Streptococcus pyogenes. CRISPR systems protect bacteria and archaea from phages by recognizing and cleaving of invading phage DNA. In practice a short gRNA is used as guide and bind the Cas9 protein to its complementary DNA strand. Cas9 protein would cleave the DNA strand at the binding site. dCas9 is the non-enzymatic variant of Cas9 form the CRISPR system. When it binds with target DNA, it cannot cause double strand breaks, which is why its name is 'dead Cas9".

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/crispr
//function/crispr/cas9
Parameters
None