Coding

Part:BBa_K2719012:Design

Designed by: Juan Carlos Rueda Silva & Arantxa Karam Coppola   Group: iGEM18_TecCEM   (2018-10-10)
Revision as of 13:13, 17 October 2018 by AKARAM (Talk | contribs) (→‎Design Notes)


TCD5 coding device with blue chromoprotein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1257


Design Notes

For tenascin was used a blue chromoprotein to improve the way of selecting colonies. For this chromoprotein was used a constitutive promoter and a double terminator. In the other case, for tenascin cassette was used a T7-lac promoter induced by IPTG. Also, it was created to be compatible with E. coli BL21 (DE3) and with pSB1C3 plasmid. (Figure 1)

"T--TecCEM--BBa_K2719010Diagram.png"

Figure 1. Diagram of BBa_K2719012, leptin expression device.

Source

TCD5 coding device with blue chromoprotein

References