Device

Part:BBa_K2719005

Designed by: Karla Soto Blas   Group: iGEM18_TecCEM   (2018-08-08)
Revision as of 12:45, 17 October 2018 by AKARAM (Talk | contribs)


Tenascin Domain V Expression Device

Tenascin was modified and optimized for Escherichia coli BL21-DE3 in order to be detected and processed by this strain. In addition, a GTS protein was fusionated to tenascine by a polylinker to conserve the three dimensional structure, because GST provides stability to the Tenascin domain and, also, is a protein that was useful for purification in the case HIS tag doesn’t worked well. This part contain a T7-lac promoter (BBa_R0184), and RBS (BBa_J611000) and a rrnB-T1 terminator (BBa_B0015) using RFC10 assembly standard, thus containing the standard BioBrick prefix and suffix.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 416


[edit]
Categories
Parameters
None