Composite

Part:BBa_K2771030:Experience

Designed by: Felipe Xavier Buson   Group: iGEM18_USP-Brazil   (2018-10-09)
Revision as of 11:51, 17 October 2018 by Felipebuson (Talk | contribs)


USP-Brazil Characterization

This part has been tested alongside with six quorum sensing promoters, pLux (BBa_K2771021), pLas (BBa_K2771022), pRhl(BBa_K2771023), pCin(BBa_K2771024), pTra (BBa_K2771025) and pRpa (BBa_K2771026). Assay in 2mL LB in 24-well plate, shaking at 180rpm and 37ºC, 2.5 mM arabinose, taking samples every 3hrs. Note that Y axis is in ratiometric activity, because reporter is measured as a ratio of YFP/CFP fluorescence:

experiment 1.
experiment 2.
Figures 1 and 2: Labels refer as Sender plasmid:Receiver plasmid, combining BBa_K2771030 with different quorum sensing promoters

We also did an assay comparing activity when induced by arabinose and when induced directly by HSL. Auto-inducing showed slower response, but much higher threshold:

experiment 3.

Applications of BBa_K2771030

User Reviews

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