Composite

Part:BBa_K2839000

Designed by: Grigorios Kyrpizidis   Group: iGEM18_Thessaloniki   (2018-10-08)
Revision as of 22:02, 15 October 2018 by Alexgiann (Talk | contribs)


TALEsp1-Pupsp1 stabilized promoter

TALE1 stabilized promoter driving sfGFP expression

Talesp1 pupsp1 Stabilized Device


Contents: Short Description Biology and Functionality Usage in our Project Illegal Sites Removal Cloning Strategy Results, Characterization Potential Applications References

1.Short description:

This part consists of a TAL Effector (TALEsp1), a TALEsp1-stabilized promoter (pupsp1) and a fluorescent marker (sfGFP). This part was originally designed by Thomas Hale Segall-Shapiro (Shapiro et al) and iGEM Thessaloniki modified it to exclude 2 PstI recognition sites, thus making it RFC[10] compatible .It can be used to achieve stabilised expression of the sfGFP marker, decoupled from gene/plasmid copy number.


Basic Part Name Registry link Type Function L2S2P21 BBa_K1401006 Terminator Terminates Transcription PT7A1w2 BBa_K2839021 Promoter Transcription Initiation SarJ BBa_K2839022 Insulator


RBSsp1 BBa_K2753036 RBS Recognized by ribosome and initiates translation TALEsp1 BBa_K2839027 Coding Sequence Binds to the PUPsp1 promoter and inhibits RNAP binding ECK-12 0029600 BBa_K2486006 Terminator Terminates Transcription PUPsp1 BBa_K2839025 Promoter Transcription Initiation RiboJ BBa_K1679042 Insulator


B0032 BBa_B0032 RBS Ribosome Binding Site sfGFP BBa_K1893032 Coding Sequence Fluorescence Marker B0015 BBa_B0015 Terminator Terminates Transcription



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2734
    Illegal XhoI site found at 3677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1707
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23


[edit]
Categories
Parameters
None