Composite

Part:BBa_K2593008

Designed by: Huimin Li   Group: iGEM18_SSTi-SZGD   (2018-09-21)
Revision as of 06:41, 9 October 2018 by Lihuimin (Talk | contribs) (→‎Reference)

P43-RBS-tuaD-gtaB-T1

This part is a functional composite part, It consists of a P43 promotor, an RBS, and an operon of tuaD-gtaB which encode UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase, respectively, to participate in the biosynthesis of HA precursor UDP- GlcUA.
Promoter P43(BBa_K1628006):P43 is a constitutive promoter that common use in Bacillus subtilis168.
RBS(BBa_K2593005): A strong ribosome binding site commonly used in Bacillus species, it is part of the pP43NMK shuttle vector.
tuaD(BBa_K1469002): tuaD is one of native gene of B. subtilis, it encodes UDP-glucose 6-dehydrogenase has 461 amino acid (also know as: UDP-GlcDH in megaterium).
gtaB(BBa_K1469005): gtaB gene encodes UTP--glucose-1-phosphate uridylyltransferase in Bacillus megaterium. The enzyme catalyzes the conversion of glucose 1-phospahate to UDP-glucose 1-phosphate.
T1 terminator(BBa_B0010):it is the most used terminator in bacteria.

T Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Peng Jin, Zhen Kang, Panhong Yuan. Production of specific-molecular-weight hyaluronan by metabolically engineered Bacillus subtilis 168[J].Metabolic Engineering, 2016:22-25

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