Part:BBa_K2886002

VEGF-scFv that can recognize the VEGF

VEGF-scFv which stands for single-chain antibody fragment of vascular endothelial growth factor is a protein domain that can recognize VEGF.

VEGF-scFv was firstly introduced to iGEM by iGEM15_NCTU-Formosa in the year 2015 (BBa_K1694003) . And this year, we make new improvement to the VEGF-scFv. In our STEP system, to improve the affinity of the VEGF-scFv to VEGF, we used Rosetta to help design our protein domain. However, we found that the initial version of VEGF-scFv in parts has no accepted structure in PDD (Protein Data Base) of NCBI. So, to improve the reliability of our results, we introduced a kind of VEGF-scFv that has accepted structure in PDD to iGEM, making it more accessible to iGEMers.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 524
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]