Coding

Part:BBa_K2789021

Designed by: Tianyi Chang   Group: iGEM18_WHU-China   (2018-10-06)
Revision as of 10:38, 6 October 2018 by Registry (Talk | contribs)

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PPN(endopolyphosphotase)

This part can catalyze the degradation of PolyP(heterochromatic granules). It is different from PPX(exopolyphosphotase) that this protein can cut the PolyP from the internal site while PPX can only cut the Polyp from the ends of Poly P, this two protein together can dramatically release the phosphorus from the bacteria. This two proteins play the opposite effect from PPK(Polyphosphokinase).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 315
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1965
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
chassisE.coli